Researchers Sequence the Entire Genome of Human Sperm
Posted on July 23, 2012
The entire genomes of 91 human sperm from one man have been sequenced by Stanford University researchers. The results are the first to report the whole-genome sequence of a human gamete, the only cells that become a child and through which parents pass on physical traits.
Stephen Quake, PhD, the Lee Otterson Professor in the School of Engineering and professor of bioengineering and of applied physics, says, "This represents the culmination of nearly a decade of work in my lab. We now have devices that will allow us to routinely amplify and sequence to a high degree of accuracy the entire genomes of single cells, which has far-ranging implications for the study of cancer, infertility and many other disorders."
Sequencing sperm cells is interesting because of a natural process called recombination that ensures that a human baby is a blend of DNA from all four of his or her grandparents. Until now, scientists had to rely on genetic studies of populations to estimate how frequently recombination had occurred in individual sperm and egg cells, and how much genetic mixing that entailed. This could change all that and give fertility researchers a new tool.
The researchers say their study shows previous, population-based estimates are, for the most part, surprisingly accurate: on average, the sperm in the sample had each undergone about 23 recombinations, or mixing events. However, individual sperm varied greatly in the degree of genetic mixing and in the number and severity of spontaneously arising genetic mutations.
Study co-author Barry Behr, PhD, HCLD, professor of obstetrics and gynecology and director of Stanford's in vitro fertilization laboratory, says, "For the first time, we were able to generate an individual recombination map and mutation rate for each of several sperm from one person. Now we can look at a particular individual, make some calls about what they would likely contribute genetically to an embryo and perhaps even diagnose or detect potential problems."
The researchers provided this explanation of the genetic recombination process in a release:
Most cells in the human body have two copies of each of 23 chromosomes, and are known as "diploid" cells. Recombination occurs during a process called meiosis, which partitions a single copy of each chromosome into a sperm (in a man) or egg (in a woman) cell. When a sperm and an egg join, the resulting fertilized egg again has a full complement of DNA.Quake says, "The exact sites, frequency and degree of this genetic mixing process is unique for each sperm and egg cell and we've never before been able to see it with this level of detail. It's very interesting that what happens in one person's body mirrors the population average."To ensure an orderly distribution during recombination, pairs of chromosomes are lined up in tight formation along the midsection of the cell. During this snug embrace, portions of matching chromosomes are sometimes randomly swapped. The process generates much more genetic variation in a potential offspring than would be possible if only intact chromosomes were segregated into the reproductive cells.
The result of the study were published here in Cell.